GeneFisher2 - Interactive PCR Primer Design

Based on the assumption that genes with related function from different organisms show high sequence similarity, degenerate primers can be designed from sequences of homologues genes. GeneFisher is an interactive web-based program for designing degenerate primers. The procedure leads to isolation of genes in a target organism using multiple alignments of related genes from different organisms. The term "gene fishing" refers to the technique where PCR is used to isolate a postulated but unknown target sequence from a pool of DNA.

Version 2.0
In the new webservice-based version 2.0 of GeneFisher, the web interface has been completely redesigned. It makes use of AJAX to provide an improved interactive behaviour to the user.

Go fishing:
To start designing primers for your "gene fishing" session, just go to the Genefisher2 Submission start page by clicking on the "Submission"-Button to the right!

If you experience problems using or have suggestions for improving the new version of GeneFisher, please contact us. If you prefer to use the old version of GeneFisher, follow this link.