Author: D. Jaeger
Welcome to
Intronserter
- a tool for
systematic integration
of
introns
into
transgenes.
This tool was developed to provide a user-friendly platform for the design
of optimized intron-containing transgenes.
Incorporation of regulatory introns into cDNA has been shown in numerous
eukaryotes to improve relative expression levels of transgenes.
In the model green microalga
Chlamydomonas reinhardtii,
spreading of introns
systematically throughout a transgene to minimize exon lengths has been
shown necessary to enable robust expression from its nuclear genome.
This webtool provides a step-by-step service for full codon optimization
and intron spreading in target transgenes to remove the guess-work from gene design.
See the
Manual
to find out how it works.
Note that gene synthesis of the optimized DNA sequence is necessary.
Please cite in your materials and methods section:
"The sequence was optimized based on the strategy
described in Baier et al, 2018, with the tool described in Jaeger et al, 2019."
(see References)
built on July 5 2019 (6:2bce081f488b)
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