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e2g [ Krüger et al.
2004] is a web-based server which efficiently
maps large expressed sequence tag (EST) and cDNA datasets to
genomic DNA. It significantly extends the volume of data that
can be mapped in reasonable time, and makes this improved
efficiency available as a web service. The server hosts large
collections of EST sequences (e.g. over 4 million mouse ESTs of
1.87 GB) in precomputed indexed data structures for efficient
sequence comparison.
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The user can upload a genomic DNA
sequence of interest and rapidly compare this to the complete
collection of ESTs on the server. This delivers a mapping of
the ESTs on the genomic DNA, allowing users to
- rapidly detect new genes
- verify the exon-intron structure of predicted genes
- determine splice variants in the uploaded genomic region of interest
The e2g webinterface provides a graphical overview of the
mapping. Alignments of the mapped EST regions with parts of the
genomic sequence are visualized. Zooming functions allow the
user to interactively explore the results. Mapped sequences can
be downloaded for further analysis. |
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